During a lumbar puncture, a needle is carefully inserted into the spinal canal low in the back. A small amount of CSF is collected, then mixed with the stem cell fluid and injected back into the CSF.
The CSF circulation (Cerebral Spinal Fluid) goes in and around the brain, separated from the blood circulation by the Blood Brain Barrier. One cycle of the CSF circulation takes between 6-7 hours. Within this time frame the stem cells will flow through the CSF into the brain.
The CSF circulation pathway body position during a lumbar puncture for stem cell injection
